Morphogenesis and development of human telencephalic organoids in the absence and presence of exogenous extracellular matrix.

ABSTRACT: The establishment and maintenance of apical-basal polarity is a fundamental step in brain development, instructing the organization of neural progenitor cells (NPCs) and the developing cerebral cortex. Particularly, basally located extracellular matrix (ECM) is crucial for this process. In vitro, epithelial polarization can be achieved via endogenous ECM production, or exogenous ECM supplementation. While neuroepithelial development is recapitulated in neural organoids, the effects of different ECM sources in tissue morphogenesis remain underexplored. Here, we show that exposure to a solubilized basement membrane matrix substrate, Matrigel, at early neuroepithelial stages causes rapid tissue polarization and rearrangement of neuroepithelial architecture. In cultures exposed to pure ECM com

SUBMITTER: Martins-Costa C 

PROVIDER: S-EPMC10646563 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature