Isolation of amide proton transfer effect and relayed nuclear Overhauser enhancement effect at -3.5ppm using CEST with double saturation powers.

ABSTRACT:

Purpose

Quantifications of amide proton transfer (APT) and nuclear Overhauser enhancement (rNOE(-3.5)) mediated saturation transfer with high specificity are challenging because their signals measured in a Z-spectrum are overlapped with confounding signals from direct water saturation (DS), semi-solid magnetization transfer (MT), and CEST of fast-exchange pools. In this study, based on two canonical CEST acquisitions with double saturation powers (DSP), a new data-postprocessing method is proposed to specifically quantify the effects of APT and rNOE.

Methods

For CEST imaging with relatively low saturation powers ( ω12$$ {\upomega}_1^2 $$ ), both the fast-exchange CEST effect and the semi-solid MT effect roughly depend on ω12$$ {\upomega}_1^2 $$ , whereas the slow-exchange AP

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC10646838 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature