Sequence-independent, site-specific incorporation of chemical modifications to generate light-activated plasmids.

ABSTRACT: Plasmids are ubiquitous in biology, where they are used to study gene-function relationships and intricate molecular networks, and hold potential as therapeutic devices. Developing methods to control their function will advance their application in research and may also expedite their translation to clinical settings. Light is an attractive stimulus to conditionally regulate plasmid expression as it is non-invasive, and its properties such as wavelength, intensity, and duration can be adjusted to minimise cellular toxicity and increase penetration. Herein, we have developed a method to site-specifically introduce photocages into plasmids, by resynthesising one strand in a manner similar to Kunkel mutagenesis. Unlike alternative approaches to chemically modify plasmids, this method is seque

SUBMITTER: Chung K 

PROVIDER: S-EPMC10646958 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature