Bright ferritin for long-term MR imaging of human embryonic stem cells.

ABSTRACT:

Background

A non-invasive imaging technology that can monitor cell viability, retention, distribution, and interaction with host tissue after transplantation is needed for optimizing and translating stem cell-based therapies. Current cell imaging approaches are limited in sensitivity or specificity, or both, for in vivo cell tracking. The objective of this study was to apply a novel ferritin-based magnetic resonance imaging (MRI) platform to longitudinal tracking of human embryonic stem cells (hESCs) in vivo.

Methods

Human embryonic stem cells (hESCs) were genetically modified to stably overexpress ferritin using the CRISPR-Cas9 system. Cellular toxicity associated with ferritin overexpression and manganese (Mn) supplementation were assessed based on cell viability, prolifer

SUBMITTER: Zhuang K 

PROVIDER: S-EPMC10647036 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature