The Complex Effects of PKM2 and PKM2:IP₃R Disruption on Intracellular Ca²⁺ Handling and Cellular Functions.

ABSTRACT: Pyruvate kinase M (PKM) 2 was described to interact with the inositol 1,4,5-trisphosphate (IP₃) receptor (IP₃R) and suppress its activity. To further investigate the physiological importance of the PKM2:IP₃R interaction, we developed and characterized HeLa PKM2 knockout (KO) cells. In the HeLa PKM2 KO cells, the release of Ca²⁺ to the cytosol appears to be more sensitive to low agonist concentrations than in HeLa wild-type (WT) cells. However, upon an identical IP₃-induced Ca²⁺ release, Ca²⁺ uptake in the mitochondria is decreased in HeLa PKM2 KO cells, which may be explained by the smaller number of contact sites between the ER and the mitochondria. Furthermore, in HeLa PKM2 KO cells, mitochondria are more numero

SUBMITTER: Lemos FO 

PROVIDER: S-EPMC10647343 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature