Registration of Functioning of a Single Horseradish Peroxidase Macromolecule with a Solid-State Nanopore.

ABSTRACT: Currently, nanopore-based technology for the determination of the functional activity of single enzyme molecules continues its development. The use of natural nanopores for studying single enzyme molecules is known. At that, the approach utilizing artificial solid-state nanopores is also promising but still understudied. Herein, we demonstrate the use of a nanotechnology-based approach for the investigation of the enzymatic activity of a single molecule of horseradish peroxidase with a solid-state nanopore. The artificial 5 nm solid-state nanopore has been formed in a 40 nm thick silicon nitride structure. A single molecule of HRP has been entrapped into the nanopore. The activity of the horseradish peroxidase (HRP) enzyme molecule inserted in the nanopore has been monitored by recording t

SUBMITTER: Ivanov YD 

PROVIDER: S-EPMC10647385 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature