Small Molecules Temporarily Induce Neuronal Features in Adult Canine Dermal Fibroblasts.

ABSTRACT: Several methods have been developed to generate neurons from other cell types for performing regeneration therapy and in vitro studies of central nerve disease. Small molecules (SMs) can efficiently induce neuronal features in human and rodent fibroblasts without transgenes. Although canines have been used as a spontaneous disease model of human central nerve, efficient neuronal reprogramming method of canine cells have not been well established. We aimed to induce neuronal features in adult canine dermal fibroblasts (ACDFs) by SMs and assess the permanency of these changes. ACDFs treated with eight SMs developed a round-shaped cell body with branching processes and expressed neuronal proteins, including βIII-tubulin, microtubule-associated protein 2 (MAP2), and neurofilament-medium. Trans

SUBMITTER: Arai K 

PROVIDER: S-EPMC10648228 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature