Comparison of in-gel and in-solution proteolysis in the proteome profiling of organ perfusion solutions.

ABSTRACT:

Purpose

The organ perfusion solution (perfusate), collected at clinically and temporally significant stages of the organ preservation and transplantation process, provides a valuable insight into the biological status of an organ over time and prior to reperfusion (transplantation) in the recipient. The objective of this study was to assess two bottom-up proteomics workflows for the extraction of tryptic peptides from the perfusate.

Experimental design

Two different kinds of perfusate samples from kidney and liver trials were profiled using liquid chromatography-mass spectrometry (LC-MS/MS). The preparation of clean peptide mixtures for downstream analysis was performed considering different aspects of sample preparation; protein estimation, enrichment, in-gel and urea-based

SUBMITTER: Snashall CM 

PROVIDER: S-EPMC10648346 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature