Ahf-Caltide, a Novel Polypeptide Derived from Calpastatin, Protects against Oxidative Stress Injury by Stabilizing the Expression of Ca_V1.2 Calcium Channel.

ABSTRACT: Reperfusion after ischemia would cause massive myocardial injury, which leads to oxidative stress (OS). Calcium homeostasis imbalance plays an essential role in myocardial OS injury. Ca_V1.2 calcium channel mediates calcium influx into cardiomyocytes, and its activity is modulated by a region of calpastatin (CAST) domain L, CS_L54-64. In this study, the effect of Ahf-caltide, derived from CS_L54-64, on myocardial OS injury was investigated. Ahf-caltide decreased the levels of LDH, MDA and ROS and increased heart rate, coronary flow, cell survival and SOD activity during OS. In addition, Ahf-caltide permeated into H9c2 cells and increased Ca_V1.2, Ca_Vβ2 and CAST levels by inhibiting protein degradation. At different Ca²⁺ concentr

SUBMITTER: Xue Y 

PROVIDER: S-EPMC10648788 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature