Global Proteome-Wide Analysis of Cysteine S-Nitrosylation in Toxoplasma gondii.

ABSTRACT: Toxoplasma gondii transmits through various routes, rapidly proliferates during acute infection and causes toxoplasmosis, which is an important zoonotic disease in human and veterinary medicine. T. gondii can produce nitric oxide and derivatives, and S-nitrosylation contributes to their signaling transduction and post-translation regulation. To date, the S-nitrosylation proteome of T. gondii remains mystery. In this study, we reported the first S-nitrosylated proteome of T. gondii using mass spectrometry in combination with resin-assisted enrichment. We found that 637 proteins were S-nitrosylated, more than half of which were localized in the nucleus or cytoplasm. Motif analysis identified seven motifs. Of these motifs, five and two contained lysine and isoleuci

SUBMITTER: Wang Z 

PROVIDER: S-EPMC10649196 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature