GroEL-Proteotyping of Bacterial Communities Using Tandem Mass Spectrometry.

ABSTRACT: Profiling bacterial populations in mixed communities is a common task in microbiology. Sequencing of 16S small subunit ribosomal-RNA (16S rRNA) gene amplicons is a widely accepted and functional approach but relies on amplification primers and cannot quantify isotope incorporation. Tandem mass spectrometry proteotyping is an effective alternative for taxonomically profiling microorganisms. We suggest that targeted proteotyping approaches can complement traditional population analyses. Therefore, we describe an approach to assess bacterial community compositions at the family level using the taxonomic marker protein GroEL, which is ubiquitously found in bacteria, except a few obligate intracellular species. We refer to our method as GroEL-proteotyping. GroEL-proteotyping is ba

SUBMITTER: Klaes S 

PROVIDER: S-EPMC10649880 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature