In Vitro Human Monoamine Oxidase Inhibition and Human Dopamine D₄ Receptor Antagonist Effect of Natural Flavonoids for Neuroprotection.

ABSTRACT: Natural flavone and isoflavone analogs such as 3',4',7-trihydroxyflavone (1), 3',4',7-trihydroxyisoflavone (2), and calycosin (3) possess significant neuroprotective activity in Alzheimer's and Parkinson's disease. This study highlights the in vitro human monoamine oxidase (hMAO) inhibitory potential and functional effect of those natural flavonoids at dopamine and serotonin receptors for their possible role in neuroprotection. In vitro hMAO inhibition and enzyme kinetics studies were performed using a chemiluminescent assay. The functional effect of three natural flavonoids on dopamine and serotonin receptors was tested via cell-based functional assays followed by a molecular docking simulation to predict interactions between a compound and the binding site of the tar

SUBMITTER: Paudel P 

PROVIDER: S-EPMC10650131 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature