Rapid Identification of High-Temperature Responsive Genes Using Large-Scale Yeast Functional Screening System in Potato.

ABSTRACT: As the third largest global food crop, potato plays an important role in ensuring food security. However, it is particularly sensitive to high temperatures, which seriously inhibits its growth and development, thereby reducing yield and quality and severely limiting its planting area. Therefore, rapid, and high-throughput screening for high-temperature response genes is highly significant for analyzing potato high-temperature tolerance molecular mechanisms and cultivating new high-temperature-tolerant potato varieties. We screened genes that respond to high temperature by constructing a potato cDNA yeast library. After high-temperature treatment at 39 °C, the yeast library was subjected to high-throughput sequencing, and a total of 1931 heat resistance candidate genes were screened. Throug

SUBMITTER: Wang K 

PROVIDER: S-EPMC10650283 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature