Genome Analysis of Thinopyrum intermedium and Its Potential Progenitor Species Using Oligo-FISH.

ABSTRACT: The genome composition of intermediate wheatgrass (IWG) is complex and continues to be a subject of investigation. In this study, molecular cytogenetics were used to investigate the karyotype composition of Th. intermedium and its relative diploid species. St₂-80 developed from Pseudowroegneria strigose and pDb12H developed from Dasypyrum breviaristatum were used as probes in fluorescence in situ hybridization (FISH) to classify the chromosomes of Th. intermedium into three groups, expressed as J^vsJ^vsJ^rJ^rStSt. A combined multiplex oligonucleotide probe, including pSc119.2-1, (GAA)₁₀, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6, was used to establish the FISH karyotype of ten accessions

SUBMITTER: Qi F 

PROVIDER: S-EPMC10650893 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature