Rice-produced classical swine fever virus glycoprotein E2 with herringbone-dimer design to enhance immune responses.

ABSTRACT: Pestiviruses, including classical swine fever virus, remain a concern for global animal health and are responsible for major economic losses of livestock worldwide. Despite high levels of vaccination, currently available commercial vaccines are limited by safety concerns, moderate efficacy, and required high doses. The development of new vaccines is therefore essential. Vaccine efforts should focus on optimizing antigen presentation to enhance immune responses. Here, we describe a simple herringbone-dimer strategy for efficient vaccine design, using the classical swine fever virus E2 expressed in a rice endosperm as an example. The expression of rE2 protein was identified, with the rE2 antigen accumulating to 480 mg/kg. Immunological assays in mice, rabbits, and pigs showed high antigenici

SUBMITTER: Xu Q 

PROVIDER: S-EPMC10651154 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature