Contractility measurements for cardiotoxicity screening with ventricular myocardial slices of pigs.

ABSTRACT:

Aims

Cardiotoxicity is one major reason why drugs do not enter or are withdrawn from the market. Thus, approaches are required to predict cardiotoxicity with high specificity and sensitivity. Ideally, such methods should be performed within intact cardiac tissue with high relevance for humans and detect acute and chronic side effects on electrophysiological behaviour, contractility, and tissue structure in an unbiased manner. Herein, we evaluate healthy pig myocardial slices and biomimetic cultivation setups (BMCS) as a new cardiotoxicity screening approach.

Methods and results

Pig left ventricular samples were cut into slices and spanned into BMCS with continuous electrical pacing and online force recording. Automated stimulation protocols were established to determine the

SUBMITTER: Shi R 

PROVIDER: S-EPMC10651213 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature