Downregulation of Heat Shock Protein 72 Contributes to Fibrostenosis in Crohn's Disease.

ABSTRACT:

Background/aims

Crohn's disease (CD) with recurrent inflammation can cause intestinal fibrostenosis due to dysregulated deposition of extracellular matrix. However, little is known about the pathogenesis of fibrostenosis. Here, we performed a differential proteomic analysis between normal, inflamed, and fibrostenotic specimens of patients with CD and investigated the roles of the candidate proteins in myofibroblast activation and fibrosis.

Methods

We performed two-dimensional difference gel electrophoresis and identified candidate proteins using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and orbitrap liquid chromatography-mass spectrometry. We also verified the levels of candidate proteins in clinical specimens and examined their effects on

SUBMITTER: Kim SW 

PROVIDER: S-EPMC10651382 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature