Rapid single-cell physical phenotyping of mechanically dissociated tissue biopsies.

ABSTRACT: During surgery, rapid and accurate histopathological diagnosis is essential for clinical decision making. Yet the prevalent method of intra-operative consultation pathology is intensive in time, labour and costs, and requires the expertise of trained pathologists. Here we show that biopsy samples can be analysed within 30 min by sequentially assessing the physical phenotypes of singularized suspended cells dissociated from the tissues. The diagnostic method combines the enzyme-free mechanical dissociation of tissues, real-time deformability cytometry at rates of 100-1,000 cells s^-1 and data analysis by unsupervised dimensionality reduction and logistic regression. Physical phenotype parameters extracted from brightfield images of single cells distinguished cell subpopulations in

SUBMITTER: Soteriou D 

PROVIDER: S-EPMC10651479 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature