Core genome multilocus sequence typing of Clostridioides difficile to investigate transmission in the hospital setting.

ABSTRACT:

Purpose

Traditional epidemiological investigations of healthcare-associated Clostridioides difficile infection (HA-CDI) are often insufficient. This study aimed to evaluate a procedure that includes secondary isolation and genomic typing of single toxigenic colonies using core genome multilocus sequence typing (cgMLST) for the investigation of C. difficile transmission.

Methods

We analyzed retrospectively all toxigenic C. difficile-positive stool samples stored at the Lausanne University Hospital over 6 consecutive months. All isolates were initially typed and classified using a modified double-locus sequence typing (DLST) method. Genome comparison of isolates with the same DLST and clustering were subsequently performed using cgMLST. The electronic administrative records of

SUBMITTER: Filippidis P 

PROVIDER: S-EPMC10651541 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature