Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa.

ABSTRACT: A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol.

SUBMITTER: Woodyer R 

PROVIDER: S-EPMC1065158 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature