Structural and dynamic mechanisms for coupled folding and tRNA recognition of a translational T-box riboswitch.

ABSTRACT: T-box riboswitches are unique riboregulators where gene regulation is mediated through interactions between two highly structured RNAs. Despite extensive structural insights, how RNA-RNA interactions drive the folding and structural transitions of T-box to achieve functional conformations remains unclear. Here, by combining SAXS, single-molecule FRET and computational modeling, we elaborate the folding energy landscape of a translational T-box aptamer consisting of stems I, II and IIA/B, which Mg²⁺-induced global folding and tRNA binding are cooperatively coupled. smFRET measurements reveal that high Mg²⁺ stabilizes IIA/B and its stacking on II, which drives the pre-docking of I and II into a competent conformation, subsequent tRNA binding promotes docking of I and II

SUBMITTER: Niu X 

PROVIDER: S-EPMC10651847 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature