Aptamer-based gold nanoparticle aggregates for ultrasensitive amplification-free detection of PSMA.

ABSTRACT: Early diagnosis is one of the most important factors in determining the prognosis in cancer. Sensitive detection and quantification of tumour-specific biomarkers have the potential to improve significantly our diagnostic capability. Here, we introduce a triggerable aptamer-based nanostructure based on an oligonucleotide/gold nanoparticle architecture that selectively disassembles in the presence of the biomarker of interest; its optimization is based also on in-silico determination of the aptamer nucleotides interactions with the protein of interest. We demonstrate this scheme for the case of Prostate Specific Membrane Antigen (PSMA) and PSMA derived from PSMA-positive exosomes. We tested the disassembly of the system by diameter and count rate measurements in dynamic light scattering, and

SUBMITTER: Matteoli G 

PROVIDER: S-EPMC10651859 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature