Characterization of the cro-ori region of the Streptococcus thermophilus virulent bacteriophage DT1.

ABSTRACT: The virulent cos-type Streptococcus thermophilus phage DT1 was previously isolated from a mozzarella whey sample, and its complete genomic sequence is available. The putative ori of phage DT1 is characterized by three inverted and two direct repeats located in a noncoding region between orf36 and orf37. As the replication ability of the putative ori and flanking genes could not be established, its ability to confer phage resistance was tested. When ori is cloned on a high-copy-number plasmid, it provides protection to S. thermophilus strains against phage infection during milk fermentation. This protection is phage specific and strain dependent. Then, a detailed transcriptional map was established for the region located between the cro-like gene (orf29) and the ori. The results of the Nort

SUBMITTER: Lamothe G 

PROVIDER: S-EPMC1065193 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature