Chitin degradation by Synechococcus WH7803.
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ABSTRACT:
SUBMITTER: Capovilla G
PROVIDER: S-EPMC10651935 | biostudies-literature | 2023 Nov
REPOSITORIES: biostudies-literature
Publications
Chitin degradation by Synechococcus WH7803.
Capovilla Giovanna G Castro Kurt G KG Collani Silvio S Kearney Sean M SM Kehoe David M DM Chisholm Sallie W SW
Scientific reports 20231115 1
Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures-yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type. ...[more]
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