HIF-1α promotes astrocytic production of macrophage migration inhibitory factor following spinal cord injury.

ABSTRACT:

Background

Macrophage migration inhibitory factor (MIF) is an important mediator of neuropathology in various central nervous system (CNS) diseases. However, little is known about its inducers for production from the nerve cells, as well as the underlying regulatory mechanism. Injury-induced HIF-1α has been shown to exacerbate neuroinflammation by activating multiple downstream target molecules. It is postulated that HIF-1α is involved in the regulation of MIF following spinal cord injury (SCI).

Methods

SCI model of Sprague-Dawley rats was established by cord contusion at T8-T10. The dynamic changes of HIF-1α and MIF protein levels at lesion site of rat spinal cord were determined by Western blot. The specific cell types of HIF-1α and MIF expression were examined by immunost

SUBMITTER: Hao H 

PROVIDER: S-EPMC10651974 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature