Zymograph profiling reveals a divergent evolution of sirtuin that may originate from class III enzymes.

ABSTRACT: Sirtuins are a group of NAD⁺-dependent deacylases that conserved in three domains of life and comprehensively involved in the regulation of gene transcription, chromosome segregation, RNA splicing, apoptosis, and aging. Previous studies in mammalian cells have revealed that sirtuins not only exist as multiple copies, but also show distinct deacylase activities in addition to deacetylation. However, the understanding of sirtuin zymographs in other organisms with respect to molecular evolution remains at an early stage. Here, we systematically analyze the sirtuin activities in representative species from archaea, bacteria, and eukaryotes, using both the HPLC assay and a 7-amino-4-methylcoumarin-based fluorogenic method. Global profiling suggests that the deacylase activities of si

SUBMITTER: Yang Y 

PROVIDER: S-EPMC10652111 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature