Experience-Induced Remodeling of the Hippocampal Post-synaptic Proteome and Phosphoproteome.

ABSTRACT: The postsynaptic density (PSD) of excitatory synapses contains a highly organized protein network with thousands of proteins and is a key node in the regulation of synaptic plasticity. To gain new mechanistic insight into experience-induced changes in the PSD, we examined the global dynamics of the hippocampal PSD proteome and phosphoproteome in mice following four different types of experience. Mice were trained using an inhibitory avoidance (IA) task and hippocampal PSD fractions were isolated from individual mice to investigate molecular mechanisms underlying experience-dependent remodeling of synapses. We developed a new strategy to identify and quantify the relatively low level of site-specific phosphorylation of PSD proteome from the hippocampus, by using a modified iTRAQ-based TiSH

SUBMITTER: Heo S 

PROVIDER: S-EPMC10652125 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature