Proteomic and Phosphoproteomic Reprogramming in Epithelial Ovarian Cancer Metastasis.

ABSTRACT: Epithelial ovarian cancer (EOC) is a high-risk cancer presenting with heterogeneous tumors. The high incidence of EOC metastasis from primary tumors to nearby tissues and organs is a major driver of EOC lethality. We used cellular models of spheroid formation and readherence to investigate cellular signaling dynamics in each step toward EOC metastasis. In our system, adherent cells model primary tumors, spheroid formation represents the initiation of metastatic spread, and readherent spheroid cells represent secondary tumors. Proteomic and phosphoproteomic analyses show that spheroid cells are hypoxic and show markers for cell cycle arrest. Aurora kinase B abundance and downstream substrate phosphorylation are significantly reduced in spheroids and readherent cells, explaining their cell c

SUBMITTER: Frederick MI 

PROVIDER: S-EPMC10652129 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature