The Pasteurella multocida nrfE gene is upregulated during infection and is essential for nitrite reduction but not for virulence.

ABSTRACT: Pasteurella multocida is the causative agent of a range of diseases with economic importance in production animals. Many systems have been employed to identify virulence factors of P. multocida, including in vivo expression technology (IVET), signature-tagged mutagenesis, and whole-genome expression profiling. In a previous study in which IVET was used with P. multocida, nrfE was identified as a gene that is preferentially expressed in vivo. In Escherichia coli, nrfE is part of the formate-dependent nitrite reductase system involved in utilizing available nitrite as an electron accepter during growth under anaerobic conditions. In this study, we constructed an isogenic P. multocida strain that was unable to reduce nitrite under either aerobic or anaerobic conditions, thereby demonstrating

SUBMITTER: Boucher DJ 

PROVIDER: S-EPMC1065219 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature