Role of the extracytoplasmic function protein family sigma factor RpoE in metal resistance of Escherichia coli.

ABSTRACT: RpoE of Escherichia coli is a sigma factor of the extracytoplasmic function protein family and is required for the expression of proteins involved in maintaining the integrity of periplasmic and outer membrane components. RpoE of E. coli was needed for full resistance to Zn(II), Cd(II), and Cu(II). Promoter gene fusion and quantitative real time reverse transcription (RT)-PCR (qRT-PCR) assays demonstrated that expression of RpoE was induced by metals. Global gene expression profiles upon metal treatment of a DeltarpoE mutant strain and its wild-type strain were analyzed with microarrays, and selected genes were confirmed by qRT-PCR. The absolute number of genes that were changed in their expression upon metal stress was similar in both strains, but the increase or decrease in transcript le

SUBMITTER: Egler M 

PROVIDER: S-EPMC1065229 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature