Senolytic treatment reduces oxidative protein stress in an aging male murine model of post-traumatic osteoarthritis.

ABSTRACT: Senolytic drugs are designed to selectively clear senescent cells (SnCs) that accumulate with injury or aging. In a mouse model of osteoarthritis (OA), senolysis yields a pro-regenerative response, but the therapeutic benefit is reduced in aged mice. Increased oxidative stress is a hallmark of advanced age. Therefore, here we investigate whether senolytic treatment differentially affects joint oxidative load in young and aged animals. We find that senolysis by a p53/MDM2 interaction inhibitor, UBX0101, reduces protein oxidative modification in the aged arthritic knee joint. Mass spectrometry coupled with protein interaction network analysis and biophysical stability prediction of extracted joint proteins revealed divergent responses to senolysis between young and aged animals, broadly sugg

SUBMITTER: Chin AF 

PROVIDER: S-EPMC10652304 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature