Transcriptome sequencing and microRNA-mRNA regulatory network construction in the lens from a Na₂SeO₃-induced Sprague Dawley rat cataract model.

ABSTRACT:

Background

A sight-threatening, cataract is a common degenerative disease of the ocular lens. This study aimed to explore the regulatory mechanism of age-related cataract (ARC) formation and progression.

Methods

Cataracts in Sprague Dawley rats were induced by adopting the method that injected selenite subcutaneously in the nape. We performed high-throughput RNA sequencing technology to identify the mRNA and microRNA(miRNA) expression profiles of the capsular membrane of the lens from Na₂SeO₃-induced and saline-injected Sprague Dawley rats. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were carried out to forecast the regulatory and functional role of mRNAs in cataracts by DAVID and Metascape. The protein-protein

SUBMITTER: Fang R 

PROVIDER: S-EPMC10652440 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature