Simvastatin induces pyroptosis via ROS/caspase-1/GSDMD pathway in colon cancer.

ABSTRACT:

Background

The outcome of patients with colon cancer is still unsatisfied nowadays. Simvastatin is a type of statins with anti-cancer activity, but its effect on colon cancer cells remains unclear. The present study is intended to determine the underlying mechanism of simvastatin in treatment of colon cancer.

Methods

The viability and pyroptosis rate of cells treated and untreated with simvastatin were analysed by CCK-8 and flow cytometry assays, respectively. We used DCFH-DA and flow cytometry to detect reactive oxygen species (ROS) production. Levels of pyroptosis markers were detected by western blotting analysis or immunofluorescence staining. Besides, the anticancer properties of simvastatin on colon cancer were further demonstrated using a cell line based xenograft tum

SUBMITTER: Xie W 

PROVIDER: S-EPMC10652480 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature