COL10A1 promotes tumorigenesis by modulating CD276 in pancreatic adenocarcinoma.

ABSTRACT:

Background

Pancreatic adenocarcinoma (PAAD) is a lethal malignant tumour. Further study is needed to determine the molecular mechanism and identify novel biomarkers of PAAD.

Methods

Gene expression data from the GSE62165 microarray were analysed with the online software Morpheus to identify differentially expressed genes (DEGs). The STRING database was used to generate a protein‒protein interaction (PPI) network for these DEGs. Hub genes were identified with Cytoscape. COL10A1 expression in PAAD was analysed via the GEPIA database. COL10A1 expression in pancreatic cancer cell lines was measured by using qRT‒PCR. The LinkedOmics database was utilized to perform survival analysis of pancreatic adenocarcinoma patients grouped based on COL10A1 expression level. CCK-8, wound heal

SUBMITTER: Xu Q 

PROVIDER: S-EPMC10652574 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature