GluCl.Cre ON enables selective inhibition of molecularly defined pain circuits.

ABSTRACT:

Abstract

Insight into nociceptive circuits will ultimately build our understanding of pain processing and aid the development of analgesic strategies. Neural circuit analysis has been advanced greatly by the development of optogenetic and chemogenetic tools, which have allowed function to be ascribed to discrete neuronal populations. Neurons of the dorsal root ganglion, which include nociceptors, have proved challenging targets for chemogenetic manipulation given specific confounds with commonly used DREADD technology. We have developed a cre/lox dependant version of the engineered glutamate-gated chloride channel (GluCl) to restrict and direct its expression to molecularly defined neuronal populations. We have generated GluCl.Cre ON that selectively renders neurons expressing cre-

SUBMITTER: Middleton SJ 

PROVIDER: S-EPMC10652717 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature