Assembling the anaerobic gamma-butyrobetaine to TMA metabolic pathway in <i>Escherichia fergusonii</i> and confirming its role in TMA production from dietary <i>L-</i>carnitine in murine models.

ABSTRACT:

Importance

The key atherosclerotic TMAO originates from the initial gut microbial conversion of L-carnitine and other dietary compounds into TMA. Developing therapeutic strategies to block gut microbial TMA production needs a detailed understanding of the different production mechanisms and their relative contributions. Recently, we identified a two-step anaerobic pathway for TMA production from L-carnitine through initial conversion by some microbes into the intermediate γBB which is then metabolized by other microbes into TMA. Investigational studies of this pathway, however, are limited by the lack of single microbes harboring the whole pathway. Here, we engineered E. fergusonii strain to harbor the whole two-step pathway and optimized the expression through

SUBMITTER: Dwidar M 

PROVIDER: S-EPMC10653785 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature