Conserved metabolic regulator ArcA responds to oxygen availability, iron limitation, and cell envelope perturbations during bacteremia.

ABSTRACT: Gram-negative facultative anaerobes often cause bacteremia, a systemic infection associated with severe clinical outcomes. ArcAB, a two-component regulatory system that represses aerobic respiration, is a key mediator of metabolic adaptation for such bacteria. Using targeted mutational analysis informed by global genetic screens, we identified the arcA gene as promoting fitness of Citrobacter freundii, Klebsiella pneumoniae, and Serratia marcescens but not Escherichia coli in a murine model of bacteremia. arcA mutants exhibit a dysregulated response to changes in oxygen availability, iron limitation, and membrane perturbations, which bacterial cells experience during infection. The genetic response of the arcA mutants to the cationic antimic

SUBMITTER: Brown AN 

PROVIDER: S-EPMC10653796 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature