Dynamic calcium-mediated stress response and recovery signatures in the fungal pathogen, Candida albicans.

ABSTRACT: Calcium (Ca²⁺) is an important second messenger for activating stress response signaling and cell adaptation in eukaryotic cells yet intracellular Ca²⁺-dynamics in fungi are poorly understood due to lack of effective real-time Ca²⁺ reporters. We engineered the GCaMP6f construct for use in the fungal pathogen, Candida albicans, and used live-cell imaging to observe both dynamic Ca²⁺ spiking and slower changes in non-spiking Ca²⁺-GCaMP signals elicited by stress or gene deletion. Short-term exposure to membrane, osmotic or oxidative stress generated immediate stress-specific responses and repeated exposure revealed differential recovery signatures. Osmotic stress caused yeast cell shrinkage and no adaptation response, where Ca

SUBMITTER: Giuraniuc CV 

PROVIDER: S-EPMC10653887 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature