Bioluminescent monitoring of recombinant lactic acid bacteria and their products.

ABSTRACT:

Importance

Lactic acid bacteria constitute a genetically diverse group of microorganisms with significant roles in the food industry, biotechnology, agriculture, and medicine. A core understanding of bacterial physiology in diverse environments is crucial to select and develop bacteria for industrial and medical applications. However, there is a lack of versatile tools to track (recombinant) protein production in lactic acid bacteria. In this study, we adapted a peptide-based bioluminescent tagging system that is functional across multiple genera and species. This system enables tracking of tagged proteins both in vitro and in situ, while it also can be used to enumerate recombinant bacteria from the mouse gastrointestinal tract with accuracy comparable to that of con

SUBMITTER: Choi IY 

PROVIDER: S-EPMC10653940 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature