Metabolic proteins with crucial roles in <i>Edwardsiella tarda</i> antioxidative adaptation and intracellular proliferation.

ABSTRACT:

Importance

Edwardsiella tarda is a significant fish pathogen that can live in challenging environments of reactive oxygen species (ROS), such as inside the phagocytes. Metabolic reconfiguration has been increasingly associated with bacterial oxidative tolerance and virulence. However, the metabolic proteins of E. tarda involved in such processes remain elusive. By proteomic analysis and functional characterization of protein null mutants, the present study identified eight crucial proteins for bacterial oxidative resistance and intracellular infection. Seven of them are metabolic proteins dictating the metabolic flux toward the generation of pyruvate, a key metabolite capable of scavenging ROS molecules. Furthermore, L-aspartate uptake, which can fuel the pyruvate gen

SUBMITTER: Wang X 

PROVIDER: S-EPMC10654080 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature