Deep mutational scanning of influenza A virus neuraminidase facilitates the identification of drug resistance mutations in vivo.

ABSTRACT:

Importance

NA is a crucial surface antigen and drug target of influenza A virus. A comprehensive understanding of NA's mutational effect and drug resistance profiles in vivo is essential for comprehending the evolutionary constraints and making informed choices regarding drug selection to combat resistance in clinical settings. In the current study, we established an efficient deep mutational screening system in mouse lung tissues and systematically evaluated the fitness effect and drug resistance to three neuraminidase inhibitors of NA single-nucleotide mutations. The fitness of NA mutants is generally correlated with a natural mutation in the database. The fitness of NA mutants is influenced by biophysical factors such as protein stability, complex formation, and the immun

SUBMITTER: Wang S 

PROVIDER: S-EPMC10654105 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature