Dopamine as a polymerizable reagent for enzyme-linked immunosorbent assay using horseradish peroxidase.

ABSTRACT: We demonstrate that dopamine can be used as a reagent for colorimetric enzyme-linked immunosorbent assay (ELISA) using horseradish peroxidase (HRP). Dopamine was able to be polymerized in the presence of HRP and H₂O₂, and black polydopamine was obtained after the enzymatic reaction. Because of the black color, the absorbance was significantly changed in the whole range of the visible light region. Here, an indirect competitive ELISA based on the polymerization of dopamine was performed to detect a fluoroquinolone antibiotic, enrofloxacin. The antibiotic is commonly used in livestock farming. The anti-antibiotics antibody was produced from egg yolk from chicken hens. In the visible range, sufficient absorbance changes of ∼0.4∼0.5 and a low background level for the ELIS

SUBMITTER: Yadoung S 

PROVIDER: S-EPMC10654240 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature