Transcriptional responses of cancer cells to heat shock-inducing stimuli involve amplification of robust HSF1 binding.

ABSTRACT: Responses of cells to stimuli are increasingly discovered to involve the binding of sequence-specific transcription factors outside of known target genes. We wanted to determine to what extent the genome-wide binding and function of a transcription factor are shaped by the cell type versus the stimulus. To do so, we induced the Heat Shock Response pathway in two different cancer cell lines with two different stimuli and related the binding of its master regulator HSF1 to nascent RNA and chromatin accessibility. Here, we show that HSF1 binding patterns retain their identity between basal conditions and under different magnitudes of activation, so that common HSF1 binding is globally associated with distinct transcription outcomes. HSF1-induced increase in DNA accessibility was modest in sca

SUBMITTER: Dastidar SG 

PROVIDER: S-EPMC10654513 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature