Isothermal chemical denaturation assay for monitoring protein stability and inhibitor interactions.

ABSTRACT: Thermal shift assay (TSA) with altered temperature has been the most widely used method for monitoring protein stability for drug research. However, there is a pressing need for isothermal techniques as alternatives. This urgent demand arises from the limitations of TSA, which can sometimes provide misleading ranking of protein stability and fail to accurately reflect protein stability under physiological conditions. Although differential scanning fluorimetry has significantly improved throughput in comparison to differential scanning calorimetry and differential static light scattering throughput, all these methods exhibit moderate sensitivity. In contrast, current isothermal chemical denaturation (ICD) techniques may not offer the same throughput capabilities as TSA, but it provides more

SUBMITTER: Mahran R 

PROVIDER: S-EPMC10654576 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature