A Combined Western and Bead-Based Multiplex Platform to Characterize Extracellular Vesicles.

ABSTRACT: In regenerative medicine, extracellular vesicles (EVs) are considered as a promising cell-free approach. EVs are lipid bilayer-enclosed vesicles secreted by cells and are key players in intercellular communication. EV-based therapeutic approaches have unique advantages over the use of cell-based therapies, such as a high biological, but low immunogenic and tumorigenic potential. To analyze the purity and biochemical composition of EV preparations, the International Society for Extracellular Vesicles (ISEV) has prepared guidelines recommending the analysis of multiple (EV) markers, as well as proteins coisolated/recovered with EVs. Traditional methods for EV characterization, such as Western blotting, require a relatively high EV sample/protein input for the analysis of one protein. We here

SUBMITTER: van Maanen JC 

PROVIDER: S-EPMC10654656 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature