Invariant NKT cells are more abundant in peanut-allergic adults and a subset of CD8⁺ iNKT cells are depleted after peanut oil exposure.

ABSTRACT:

Introduction

Peanut allergy is one of the most prevalent food allergies globally. Currently, most research into the mechanisms involved in protein allergy focuses on the protein allergens under investigation, and information on the function of accompanying compounds, such as lipids, is scarce. Thus, this research investigates the role of peanut-associated lipids and invariant natural killer T (iNKT) cells in peanut allergy using a novel, human, in vitro assay.

Methods

PBMCs from non-allergic and peanut-allergic subjects were stimulated with the glycolipid, α-Galactosylceramide (α-GalCer), over 14 days for iNKT cell expansion. Autologous dendritic cells (DCs) were stimulated with either peanut oil, the lipid-binding peanut allergen, Ara h 8, or both peanut oil and Ara

SUBMITTER: Hopkins GV 

PROVIDER: S-EPMC10655100 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature