Cell-Surface-Retained Peptide Additives for the Cytosolic Delivery of Functional Proteins.

ABSTRACT: The delivery of functional proteins remains a major challenge in advancing biological and pharmaceutical sciences. Herein, we describe a powerful, simple, and highly effective strategy for the intracellular delivery of functional cargoes. Previously, we demonstrated that cell-penetrating peptide (CPP) additives equipped with electrophilic thiol-reactive moieties temporarily attach to the cellular membrane, thereby facilitating the cellular uptake of protein- and antibody-CPP cargoes through direct membrane transduction at low concentrations. Now, we hypothesize that CPP-additives with an increased retention on the cellular membrane will further enhance intracellular uptake. We discovered that adding a small hydrophobic peptide sequence to an arginine-rich electrophilic CPP-additive further

SUBMITTER: Arafiles JVV 

PROVIDER: S-EPMC10655119 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature