Culture-dependent screening of endospore-forming clostridia in infant feces.

ABSTRACT:

Background

Only a few studies dealt with the occurrence of endospore-forming clostridia in the microbiota of infants without obvious health complications.

Methods

A methodology pipeline was developed to determine the occurrence of endospore formers in infant feces. Twenty-four fecal samples (FS) were collected from one infant in monthly intervals and were subjected to variable chemical and heat treatment in combination with culture-dependent analysis. Isolates were identified by MALDI-TOF mass spectrometry, 16S rRNA gene sequencing, and characterized with biochemical assays.

Results

More than 800 isolates were obtained, and a total of 21 Eubacteriales taxa belonging to the Clostridiaceae, Lachnospiraceae, Oscillospiraceae, and Peptostreptococcaceae families were detec

SUBMITTER: Ingribelli E 

PROVIDER: S-EPMC10655253 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature