WGX50 mitigates doxorubicin-induced cardiotoxicity through inhibition of mitochondrial ROS and ferroptosis.

ABSTRACT:

Background

Doxorubicin (DOX)-induced cardiotoxicity (DIC) is a major impediment to its clinical application. It is indispensable to explore alternative treatment molecules or drugs for mitigating DIC. WGX50, an organic extract derived from Zanthoxylum bungeanum Maxim, has anti-inflammatory and antioxidant biological activity, however, its function and mechanism in DIC remain unclear.

Methods

We established DOX-induced cardiotoxicity models both in vitro and in vivo. Echocardiography and histological analyses were used to determine the severity of cardiac injury in mice. The myocardial damage markers cTnT, CK-MB, ANP, BNP, and ferroptosis associated indicators Fe²⁺, MDA, and GPX4 were measured using ELISA, RT-qPCR, and western blot assays. The morphology of mitocho

SUBMITTER: Tai P 

PROVIDER: S-EPMC10655295 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature