TIGIT reverses IFN-α-promoted Th1-like Tregs via in-sequence effects dependent on STAT4.

ABSTRACT:

Objectives

The induction direction of interferon (IFN)-α in T-cell phenotype and function varies depending on the activation state of the cell and the time of stimulation. To assess the effects of elevated IFN-α on regulatory T cells (Tregs) in systemic lupus erythematosus (SLE) patients, we investigated the differentiation of Th1-like Tregs under in-sequence and out-of-sequence conditions and the reversal effect of activating TIGIT on immune suppression.

Methods

Phenotypes and activation levels of Tregs from SLE patients and healthy controls were analyzed using flow cytometry. In vitro culture conditions based on the sequence of TCR activation and IFN-α stimulation simulated in-sequence or out-of-sequence effects. CD4⁺T cells and Tregs were cultured under the abo

SUBMITTER: Yu S 

PROVIDER: S-EPMC10655484 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature